PRELIMINARY EXAMINATION OF STEROID IN COMMONLY AVAILABLE UNREGISTERED, APHRODIASIC AND ANTI-ARTHERITIC HERBAL FORMULATIONS

PRELIMINARY EXAMINATION OF STEROID IN COMMONLY AVAILABLE UNREGISTERED, APHRODIASIC AND ANTI-ARTHERITIC HERBAL FORMULATIONS

Abstraction

The current survey is designed to measure the presence of steroid in normally used unregistered sexy and anti-arthritic herbal preparation because in unregistered herbal preparations. Steroids are added intentionally to give rapid curative effects due to miss of quality control where as the container of the drug indicate names of herbs which are really non added in these unregistered preparations. Although the work is done on preliminary degree but this work will open the door for research workers to measure unregistered herbal preparations for the presence of phyto steroid or steroids which are added in preparation for rapid curative effects.

Keywords:

Unregistered drug, poly herbal preparation, sexy, anti-arthritis, debasement.

Introduction:

Herbalism is the most popular signifier of traditional medical specialty due to their effectivity, less inauspicious effects, low monetary values and easy handiness. Most of the herbal merchandises sold in community pharmaceutics and wellness nutrient stores are non registered hence missing readily accessible cardinal safety information ( cusps ) . Many herbal merchandises are insecure for usage by people with some preexistent unwellnesss for illustration Asian ginseng is contra-indicated for diabetes ; interaction with other merchandises for illustration the usage of St John’s Wort impacting the efficaciousness of Coumadin ; inauspicious effects for illustration allergic reactions are associated with gingko. Unlicensed herbal redresss are broad spread due to the deficiency of pharmacovigilence.These redresss don’t meet specific criterions of safety and quality. ( Raynoret Al, 2011 ) .

Herbal drug use in Pakistan is really popular. These drugs are non merely use in modern medical specialties but in the signifier of rough medicative workss, poly herbal preparations and herbal infusions has been used in Unani, Ayurvedic and Homeopathic system of medical specialty. The presented work was designed to measure some normally available unregistered aphrodisiac and anti-arthritic herbal preparations for the presence of steroid.

MATERIALS AND METHODS

Normally used unregistered aphrodisiac and anti creaky poly herbal preparations were selected ( 10 different samples of each class ) . Each herbal preparation was marked for the codification figure assigned to each herbal preparation.

Qualitative analysis

The Crude infusion was tested for the presence of steroid

compounds in Formulation by utilizing following standard methods.

Trial for Steroids:

Salkowski’s trial

Crude infusion was assorted with 2ml of trichloromethane. Then 2ml of concentrated H2SO4 was added carefully and agitate gently. A ruddy brown coloring material indicated the presence of steroidal ring, i.e. , glycone part of the glycoside. [ 14 ]

Liebermann Burchard trial

Crude infusion was assorted with few beads of acetic anhydride, boiled and cooled. Concentrated sulfuric acid was so added from the sides of the trial tubing and observed for the formation of a brown ring at the junction of two beds. Green colour of the upper bed and the formation of deep ruddy colour in the lower bed would bespeak a positive trial for steroids and triterpenoids severally. [ 11

Table 1.

Merchandises Code	Salkowski Trial	Liebermann Trial	Merchandises Code	Salkowski Trial	Liebermann Trial
APF 1	+ ve	+ ve	AAF 1	– ve	– ve
APF 2	+ ve	+ ve	AAF 2	+ ve	+ ve
APF 3	+ ve	+ ve	AAF 3	– ve	– ve
APF 4	+ ve	+ ve	AAF 4	+ ve	+ ve
APF 5	+ ve	+ ve	AAF 5	+ ve	+ ve
APF 6	+ ve	+ ve	AAF 6	+ ve	+ ve
APF 7	+ ve	+ ve	AAF 7	+ ve	+ ve
APF 8	– ve	– ve	AAF 8	+ ve	+ ve
APF 9	– ve	– ve	AAF 9	+ ve	+ ve
APF 10	+ ve	+ ve	AAF 10	+ ve	+ ve
APF = Aphrodisiac Herbal Formulation ; AAF =Anti Arthritic Herbal Formulation ; +ve = Presence of Steroid ; -ve = Absence of Steroid ; N = 3.

Table 2.The mentioned composing of used aphrodisiac and anti-arthritic poly herbal preparations.

Merchandises Code	Composition	Merchandises Code	Composition
APF 1	Not mentioned	AAF 1	Rhus toxD3, Dexa, Arnica D3, Caulophyllum D3, Urtica
APF 2	Not mentioned	AAF 2	Belispers,Calocenth, Hypericum, satesorenjan, Satehanzal
APF 3	Agnus cast, Selenium, Sabal ser, Yohambinum, Ginseng, Acid phos	AAF 3	Rhus tox, Bryonia D3, Urtica urtens D3,Belladona, Ginger
APF 4	Not mentioned	AAF 4	Dulcamara, Guicum,Rhus tox,Belladona, Bryonia
APF 5	Ocimumbasilicum, Mace, Nutmegs, Zagar, Cloves, Silk,Hyosamus	AAF 5	Ledum,Arnica,Colocynthis, Urtica Ur,Rhustox
APF 6	Agnus cast, Acid phos, Sabal ser, Damiana, Bombyx mori	AAF 6	Acid benz D3, Bryonia D3, Urtica urtens D3,Caulophyllum
APF 7	Lycopodium,Avena sativa, Ambra, Nuphar lut,Orchitinum	AAF 7	Arnica, Ruta, Bryonia, Rhus tox
APF 8	Ginseng, Ambra, Acid phos, Helonias diocia,Allium sativum	AAF 8	Dexa, Urtica ur D3, Caulophyllum D3,Belladona
APF 9	Not mentioned	AAF 9	Not mentioned
APF 10	Kalium phos,Argentum nitricum, Uva ursi, Cantharis, Sabal serrulata	AAF 10	Not mentioned

LIEBERMANN’S TrialSALKOWSKI’S Trial

RESULTS AND DISCUSSION:

The observations of tried herbal preparations and their composing are mentioned in table 1 and 2. Most of the tried samples indicate the presence of steroid. Results of this research survey will be helpful for making consciousness non merely to the ballad individual but besides to herbal practicians and doctors about the presence of steroids in unaccredited poly herbal preparations and farther more boycott of these unregistered herb tea preparations for farther ingestion.

The populace should be able to anticipate full revelation of cardinal safety information when they purchase herbal merchandises over the counter. Consumers need to be advised what are registered merchandises, where and how to buy these merchandises. So, that the necessary information for safe usage will be available to them. In add-on there should be on traveling monitoring of the written information supplied with these merchandises to promote betterment in this of import safety issue ( Raynoret Al, 2011 ) .

There are four possibilities of forgery in herbal medical specialties. The first 1 is herbal medical specialties adulterated with undeclared man-made pharmaceuticals, with undeclared heavy metals, herbal medical specialties without active ingredient and herbal medical specialties with incorrect active ingredient [ Herbal Remedies ] .

Awareness should be made to the general populace, local therapists and the doctors about the toxicities of steroids to human wellness.

The presented work is of preliminary degree. However most reliable pharmacopeial methods are required to corroborate the presence of steroids in tried sample every bit good as methods to measure the steroids of added herbs or other externally added steroids.

In modern epoch, patients are still utilizing drugs prescribed by quacks and 21 % of Quackery Formulation in Rawalpindi is contaminated with steroids. The steroids comprise chiefly of Decadron and Pediapred in debased tablets and pulverizations prescribed by hakims. Many quacks contaminate drugs with steroids for early alleviation of symptoms, although they do non label their preparations or bespeak the steroid content ( phuaet Al, 2009 ; koishnaparsadet Al, 2005 ) .

In Pakistan a major proportion of population is un-educated and there is a deficiency of proper medical attention installations even in the metropoliss for hapless patients. These people take the empiricism preparations from different beginnings. Because of the deficiency of comprehensive ordinances sing empiricism patterns and criterions for empiricism preparations, it is imperative that these drugs be screened for steroids with an purpose to protect the patients from inauspicious effects of these contaminations ( Attardeet Al, 2008 ; Ciesla & A ; wadmunzka,2009 ) .

Decision:

Mentions:

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